Contact an Account ManagerSimple method for the determination of morphine and its active glucuronide metabolite in human plasma
Summary
Type: HPLC
Application #: 101250
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: electrochemical, potentials +0.35V, +0.45V and +0.55V for detector 1, detector 2 and the guard cell, respectively
Conditions: eluent: 10mM NaH2PO4, 1mM Na dodecyl sulphate (SDS), 26% (v/v) acetonitrile, adjusted to pH 2.1 with o-phosphoric acid,
flow rate 1.5ml/min
Authors: Mason J L
Source: J Chromatogr 570 (1991) 191 - 197
Details
Legend: Chromatogram showing recovery of 50nmol/l morphine (M) and
morphine-6-glucuronide (M6G) standard from plasma:
A) aqueous standard,
B) plasma blank,
C) spiked plasma.